电离辐射通过转变成生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简短 ：探讨辐射源是否可通过转化生长因子-β（TGF-β）-内源性的结缔一组织-上皮细胞反转 (EMT)来增进骨髓的肆虐迁离。使用分之一2Gy(60)Coγ线紫外光源自人类肝脏的6种骨髓，记录与EMT特别的变化，这以外分别并用成像技术，核糖体印迹方规，免疫荧光技术，划痕试验中和Transwell四人试验中来观察并检测细胞一组织有机体，EMT标识，肆虐迁离能够等。改用酶联免疫吸附规检测这些骨髓之中TGF-β酶程度，并用特别抑制剂SB431542来评估TGF-β频率移动式在辐射源EMT之中的功用。经过分之一为2Gy紫外光的骨髓之中存在间叶细胞的表达，与所谓紫外光一组相对来说其结缔一组织标识减少，间叶细胞标识减低，同时其肆虐移往能够增强，TGF-β酶程度也更高。进一步发现由A549辐射源游离的EMT可通过对TGF-β频率抑制发生逆转。这些结果表明TGF-β内源性的EMT在辐射源游离增强骨髓肆虐移往能够之中起着关键功用。

校对： lizexiu